Confocal Microscopy

Confocal microscopy enables acquisition of three-dimensionally resolved fluorescence images by collecting emissions only from the focus of a scanned laser beam.  Our state-of-the-art confocal microscopes have tremendous flexibility in terms of excitation laser lines, emission color, automated sampling protocols, and environmental controls.  Point-scanning confocals, like our Zeiss LSM710 and Leica SP2 enable the greatest spectral resolution, image zoom and optical sectioning quality.  The Andor Revolution spinning disk microscope offers the capability for extremely fast acquisition (~100 images/sec), with the potential for reduced phototoxicity.

Confocal microscopy can be used to locate fluorescent objects with sub-micron resolution in live or fixed cells, engineered or cultured tissues, plants, materials or devices.  Typical applications include GFP localization and tracking, immunohistochemistry, organelle staining and nanoparticle targeting.  Molecular association can be assessed by multiple colored labels, using FRET or co-localization analyses.  Dynamics can be imaged and measured using photobleaching and/or photoconversion of appropriate fluorophores.  Though fluorescence is the primary imaging mode, reflected light confocal microscopy can be used to image collagen fibrils or other material properties.
Samples can be fixed or live, sectioned or unsectioned, stained or unstained depending on the application.  After an individual training and consultation session (contact), researchers are given access to schedule instrument time.    Assistance with imaging and experimental design is available during normal business hours.